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Binding of transcription factors measured on individual DNA molecules in living cells. An important question in the field of gene regulation is how a limited set of transcription factors interpret the same genome in different ways to create over cell types found in our bodies, all of which are defined by the expression of a unique set of genes. To achieve this, transcription factors have evolved cooperative mechanisms to enable precise control of gene expression. In a new study, members of the Krebs group in the Genome Biology Unit at EMBL Heidelberg have developed a method to directly visualise how transcription factors co-occupy DNA in living cells, and have used it to better understand their cooperativity mechanisms.
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